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Technical Data: Size Exclusion Chromatography (GPC) Handbook

A detailed explanation of SEC (GPC) used for evaluating the molecular weight of polymers! (For SEC of proteins, please refer to the "Bioseparation Handbook.")

This document is a technical material that explains size exclusion chromatography (SEC). We will introduce the basic principles of SEC, measurement methods, and examples of measurements of actual samples. [Contents (excerpt)] - Physical properties and molecular weight of polymers, molecular weight distribution - Measurement methods for average molecular weight and molecular weight distribution - Molecular weight measurement using SEC - Column selection methods - Elution solvents - Sample concentration and injection volume - SEC measurement of super high molecular weight - High-temperature SEC *For more details, please refer to the PDF document or feel free to contact us.

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【Technical Data】UHPL-SEC column with reduced sample adsorption!

The UHPL-SEC column that suppresses sample adsorption is now decided! This is a must-see for everyone struggling with initial adsorption in SEC columns.

This is a technical document explaining the features of the new TOSOH SEC column "TSKgel UP-SW3000-LS," which addresses the phenomenon of non-specific adsorption of proteins (initial adsorption) often observed when equilibrating a new size exclusion chromatography (SEC) column with elution buffer and injecting protein samples. This phenomenon is particularly prevalent with antibody samples, where aggregates or dimers may adsorb, leading to a reduction in elution peaks or, in some cases, no peaks being eluted at all. Such occurrences are seen in nearly all SEC columns.

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